Hotspot

trnK-UUU-rps16

LSC · 4359-5274 · 916 bp

Back to Sambucus

Pi 0.00935

MarkerSeek score 65.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 916
variable_sites: 29
pi: 0.00935
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCCCTCGCTTCATCCAAATG	TTGAAAAAGGTGCTCAGCCT	50.0	45.0	58.0	57.9	1054-1098 bp	1.0	85.3
trnK-UUU-rps16_p2	TCCCTCGCTTCATCCAAATG	AAAAAGGTGCTCAGCCTACC	50.0	50.0	58.0	58.1	1051-1095 bp	1.0	85.1
trnK-UUU-rps16_p3	CCGATGGTATGGACGAATCC	TTGAAAAAGGTGCTCAGCCT	55.0	45.0	57.9	57.9	1071-1115 bp	1.0	85.0
trnK-UUU-rps16_p4	CGATGGTATGGACGAATCCC	TTGAAAAAGGTGCTCAGCCT	55.0	45.0	57.9	57.9	1070-1114 bp	1.0	85.0
trnK-UUU-rps16_p5	CGATGGTATGGACGAATCCC	AAAAAGGTGCTCAGCCTACC	55.0	50.0	57.9	58.1	1067-1111 bp	1.0	84.8