Hotspot

tRNA-Leu-tRNA-Asn

SSC · 109712-130234 · 20523 bp

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Pi 0.105

MarkerSeek score 80.80

Primer available yes

Label tRNA-Leu-tRNA-Asn

Feature Metadata

label_name: tRNA-Leu-tRNA-Asn
feature_type: igs
region: SSC
length_bp: 20523
variable_sites: 2103
pi: 0.105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Leu-tRNA-Asn_p1	GGCCTGCCTTTTTGCATAAG	AAAGGAATCGCATACGGCAT	50.0	45.0	58.0	58.0	795 bp	0.5	59.6
tRNA-Leu-tRNA-Asn_p2	GGCCTGCCTTTTTGCATAAG	AAGGAATCGCATACGGCATT	50.0	45.0	58.0	58.0	794 bp	0.5	59.6
tRNA-Leu-tRNA-Asn_p3	GGCCTGCCTTTTTGCATAAG	AGGAATCGCATACGGCATTT	50.0	45.0	58.0	58.0	793 bp	0.5	59.6
tRNA-Leu-tRNA-Asn_p4	GGCCTGCCTTTTTGCATAAG	TATGAGGGGGTTCTCTGCAA	50.0	50.0	58.0	58.0	872 bp	0.5	59.6
tRNA-Leu-tRNA-Asn_p5	GGCCTGCCTTTTTGCATAAG	TTATGAGGGGGTTCTCTGCA	50.0	50.0	58.0	58.0	873 bp	0.5	59.6