Hotspot

tRNA-Phe-tRNA-Val

LSC · 48119-50489 · 2371 bp

Back to Salvinia

Pi 0.0655

MarkerSeek score 78.40

Primer available yes

Label tRNA-Phe-tRNA-Val

Feature Metadata

label_name: tRNA-Phe-tRNA-Val
feature_type: igs
region: LSC
length_bp: 2371
variable_sites: 149
pi: 0.0655
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Phe-tRNA-Val_p1	GTTCAAGTCTGGTTCCTGGC	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.8	57.9	2347-2414 bp	1.0	81.5
tRNA-Phe-tRNA-Val_p2	GTTCAAGTCTGGTTCCTGGC	CCGAGATTGTCTACGGTTCG	55.0	55.0	58.8	58.2	2361-2428 bp	1.0	80.7
tRNA-Phe-tRNA-Val_p3	AGTTCAAGTCTGGTTCCTGG	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.1	57.9	2348-2415 bp	1.0	80.1
tRNA-Phe-tRNA-Val_p4	CCAGTTCAAGTCTGGTTCCT	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.1	57.9	2350-2417 bp	1.0	80.1
tRNA-Phe-tRNA-Val_p5	GTTCAAGTCTGGTTCCTGGC	TCTGCATGACCGAGATTGTC	55.0	50.0	58.8	57.7	2370-2437 bp	1.0	79.6