Hotspot

tRNA-Leu-tRNA-Phe

LSC · 47698-48045 · 348 bp

Back to Salvinia

Pi 0.158

MarkerSeek score 78.20

Primer available yes

Label tRNA-Leu-tRNA-Phe

Feature Metadata

label_name: tRNA-Leu-tRNA-Phe
feature_type: igs
region: LSC
length_bp: 348
variable_sites: 51
pi: 0.158
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Leu-tRNA-Phe_p1	GTGACAGCTCCCAGATTCAG	GAAGAGTGGCTGTCAGTAGC	55.0	55.0	58.0	58.0	1451-1468 bp	1.0	88.8
tRNA-Leu-tRNA-Phe_p2	GTAGACGCAACGGACTCAAT	GAAGAGTGGCTGTCAGTAGC	50.0	55.0	58.0	58.0	1502-1519 bp	1.0	88.6
tRNA-Leu-tRNA-Phe_p3	GTAGACGCAACGGACTCAAT	GTGGTTTCAATTACCTGCGC	50.0	50.0	58.0	58.0	1465-1482 bp	1.0	88.6
tRNA-Leu-tRNA-Phe_p4	GTAGACGCAACGGACTCAAT	CGTCTAGCTCCTTGTCCAAC	50.0	55.0	58.0	58.0	1020 bp	0.5	59.9
tRNA-Leu-tRNA-Phe_p5	GTGACAGCTCCCAGATTCAG	CGTCTAGCTCCTTGTCCAAC	55.0	55.0	58.0	58.0	969 bp	0.5	59.8