Hotspot

tRNA-Ser-tRNA-Leu

LSC · 45434-47186 · 1753 bp

Back to Salvinia

Pi 0.0906
MarkerSeek score 79.70
Primer available yes
Label tRNA-Ser-tRNA-Leu

Feature Metadata

label_name
tRNA-Ser-tRNA-Leu
feature_type
igs
region
LSC
length_bp
1753
variable_sites
156
pi
0.0906
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Ser-tRNA-Leu_p1 ATTGACTACCGGCTTGTCAC ATTGAGTCCGTTGCGTCTAC 50.0 50.0 57.9 58.0 1951-1952 bp 1.0 87.1
tRNA-Ser-tRNA-Leu_p2 ACTACCGGCTTGTCACTTTG ATTGAGTCCGTTGCGTCTAC 50.0 50.0 58.1 58.0 1947-1948 bp 1.0 86.9
tRNA-Ser-tRNA-Leu_p3 ATTGACTACCGGCTTGTCAC CTGAATCTGGGAGCTGTCAC 50.0 55.0 57.9 58.0 2002-2003 bp 1.0 86.7
tRNA-Ser-tRNA-Leu_p4 ATTGACTACCGGCTTGTCAC GTTGAGATGCAACGGAAAGC 50.0 50.0 57.9 58.0 2269-2270 bp 1.0 86.6
tRNA-Ser-tRNA-Leu_p5 ACTACCGGCTTGTCACTTTG CTGAATCTGGGAGCTGTCAC 50.0 55.0 58.1 58.0 1998-1999 bp 1.0 86.5