Hotspot

tRNA-Ser-tRNA-Thr

LSC · 32174-36769 · 4596 bp

Back to Salvinia

Pi 0.0689

MarkerSeek score 72.60

Primer available yes

Label tRNA-Ser-tRNA-Thr

Feature Metadata

label_name: tRNA-Ser-tRNA-Thr
feature_type: igs
region: LSC
length_bp: 4596
variable_sites: 289
pi: 0.0689
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ser-tRNA-Thr_p1	TCTCCTTGCAATACTCACGC	AGGGCCAGTATGTTTTGTGA	50.0	45.0	58.0	57.0	796 bp	0.5	52.2
tRNA-Ser-tRNA-Thr_p2	TCGTATCGAATCCTCAGGCT	AGGGCCAGTATGTTTTGTGA	50.0	45.0	58.0	57.0	418 bp	0.5	52.1
tRNA-Ser-tRNA-Thr_p3	CGGCCTCGATCAGTAAGAAG	AGGGCCAGTATGTTTTGTGA	55.0	45.0	57.9	57.0	684 bp	0.5	51.2
tRNA-Ser-tRNA-Thr_p4	GGCCTCGATCAGTAAGAAGC	AGGGCCAGTATGTTTTGTGA	55.0	45.0	58.1	57.0	683 bp	0.5	51.1
tRNA-Ser-tRNA-Thr_p5	ACTACTGACTGGATGAGCCA	AGGGCCAGTATGTTTTGTGA	50.0	45.0	57.8	57.0	262 bp	0.5	50.3