Hotspot

tRNA-Ser-tRNA-Gly

LSC · 9330-10153 · 824 bp

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Pi 0.0783

MarkerSeek score 81.00

Primer available yes

Label tRNA-Ser-tRNA-Gly

Feature Metadata

label_name: tRNA-Ser-tRNA-Gly
feature_type: igs
region: LSC
length_bp: 824
variable_sites: 63
pi: 0.0783
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ser-tRNA-Gly_p1	ATTAGCAATCCGACGCGTTA	TACCACTAAACCATACCCGC	45.0	50.0	57.8	57.0	869-884 bp	1.0	77.9
tRNA-Ser-tRNA-Gly_p2	TTAGACCTCTCGGCCATCTC	TACCACTAAACCATACCCGC	55.0	50.0	58.3	57.0	852-867 bp	1.0	77.3
tRNA-Ser-tRNA-Gly_p3	TTAGCAATCCGACGCGTTAG	TACCACTAAACCATACCCGC	50.0	50.0	58.4	57.0	868-883 bp	1.0	76.0
tRNA-Ser-tRNA-Gly_p4	ATTAGCAATCCGACGCGTTA	TTACCACTAAACCATACCCGC	45.0	47.6	57.8	57.7	870-885 bp	1.0	75.7
tRNA-Ser-tRNA-Gly_p5	TTAGACCTCTCGGCCATCTC	TTACCACTAAACCATACCCGC	55.0	47.6	58.3	57.7	853-868 bp	1.0	75.1