Hotspot

ycf1

SSC · 120975-126500 · 5526 bp

Pi 0.0321

MarkerSeek score 70.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5526
variable_sites: 1277
pi: 0.0321
unique_haplotype_count: 100
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTATCCCAGGCCTCTGCTAT	AAGAGGGATCCACCGAAGAA	55.0	50.0	58.1	58.0	1492-1607 bp	0.971429	90.1
ycf1_p2	TATCCCAGGCCTCTGCTATC	AAGAGGGATCCACCGAAGAA	55.0	50.0	58.1	58.0	1491-1606 bp	0.971429	90.0
ycf1_p3	CTATCCCAGGCCTCTGCTAT	ACAGGAACAAGAGGGATCCA	55.0	50.0	58.1	58.0	1500-1615 bp	0.961905	89.7
ycf1_p4	TATCCCAGGCCTCTGCTATC	ACAGGAACAAGAGGGATCCA	55.0	50.0	58.1	58.0	1499-1614 bp	0.961905	89.7
ycf1_p5	CTATCCCAGGCCTCTGCTAT	GACGCGTTTCTGAAGAGTCT	55.0	50.0	58.1	57.9	1342-1457 bp	0.961905	89.6