Hotspot

ndhG-ndhI

SSC · 115962-116324 · 363 bp

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Pi 0.0551
MarkerSeek score 73.00
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
363
variable_sites
124
pi
0.0551
unique_haplotype_count
73
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 ATTGGTCCGGGTAAATCCAT TCGAATTTACCTCAACTCAACA 45.0 36.4 56.6 55.7 168-442 bp 0.990476 51.6
ndhG-ndhI_p2 GTATTGGTCCGGGTAAATCCA TCGAATTTACCTCAACTCAACA 47.6 36.4 57.4 55.7 170-444 bp 0.990476 51.6
ndhG-ndhI_p3 TGTATTGGTCCGGGTAAATCC TCGAATTTACCTCAACTCAACA 47.6 36.4 57.4 55.7 171-445 bp 0.990476 51.6
ndhG-ndhI_p4 ATTGGTCCGGGTAAATCCAT CTTCGAATTTACCTCAACTCAACA 45.0 37.5 56.6 57.5 170-444 bp 0.990476 51.6
ndhG-ndhI_p5 ATTGGTCCGGGTAAATCCAT TCGAATTTACCTCAACTCAACAA 45.0 34.8 56.6 56.4 168-442 bp 0.990476 51.6