Hotspot

rpl32-trnL-UAG

SSC · 110741-111388 · 648 bp

Back to Salvia

Pi 0.0549
MarkerSeek score 78.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
648
variable_sites
198
pi
0.0549
unique_haplotype_count
85
primer_available
yes
alignment_reliability
0.98
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGGTTGCTGTCGGAAAAAGA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.0 57.9 1046-1522 bp 0.933333 88.0
rpl32-trnL-UAG_p2 TGGTTGCTGTCGGAAAAAGA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.0 57.8 1396-1515 bp 0.780952 81.4
rpl32-trnL-UAG_p3 TGGTTGCTGTCGGAAAAAGA AGATGAAATGTCATGCCGCT 45.0 45.0 58.0 58.0 1462-1486 bp 0.171429 50.4
rpl32-trnL-UAG_p4 CATCCCGCTATACCAACGAG AGATGAAATGTCATGCCGCT 55.0 45.0 57.9 58.0 952-972 bp 0.161905 48.7
rpl32-trnL-UAG_p5 CATCCCGCTATACCAACGAG TCCTAAGAGCAGCGTGTCTA 55.0 50.0 57.9 57.9 898-918 bp 0.161905 48.3