Hotspot

trnT-GGU-psbD

LSC · 30545-31916 · 1372 bp

Back to Salvia

Pi 0.0269
MarkerSeek score 74.80
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1372
variable_sites
279
pi
0.0269
unique_haplotype_count
89
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG TTTACCAAGGGCTATAGTCA 55.0 40.0 58.1 53.0 1316-1467 bp 0.990476 51.6
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.1 53.0 1319-1470 bp 0.990476 51.6
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.3 53.0 1293-1444 bp 0.990476 51.6
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG TTACCAAGGGCTATAGTCAT 55.0 40.0 58.1 52.8 1315-1466 bp 0.990476 51.6
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA TTACCAAGGGCTATAGTCAT 50.0 40.0 58.1 52.8 1318-1469 bp 0.990476 51.6