Hotspot

trnE-UUC-trnT-GGU

LSC · 29932-30472 · 541 bp

Back to Salvia

Pi 0.0355
MarkerSeek score 72.50
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
541
variable_sites
110
pi
0.0355
unique_haplotype_count
63
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 623-687 bp 0.990476 89.5
trnE-UUC-trnT-GGU_p2 GCTGCCTCCTTGAAAGAGAG CCATGGCGTTACTCTACCAC 55.0 55.0 58.3 58.1 602-666 bp 0.990476 89.0
trnE-UUC-trnT-GGU_p3 GCTGCCTCCTTGAAAGAGAG TGGCGTTACTCTACCACTGA 55.0 50.0 58.3 58.1 599-663 bp 0.990476 88.8
trnE-UUC-trnT-GGU_p4 GCTGCCTCCTTGAAAGAGAG CCGATGACTTACGCCTTACC 55.0 55.0 58.3 58.2 620-684 bp 0.990476 88.3
trnE-UUC-trnT-GGU_p5 GCTGCCTCCTTGAAAGAGAG TTGAACCGATGACTTACGCC 55.0 50.0 58.3 58.3 625-689 bp 0.990476 87.2