Hotspot

petN-psbM

LSC · 28057-29043 · 987 bp

Back to Salvia

Pi 0.0321
MarkerSeek score 71.70
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
987
variable_sites
233
pi
0.0321
unique_haplotype_count
88
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCGTAGTGTGGGGAAGAAGT CGGCGGGTTGATTTGATAGA 50.0 50.0 58.0 58.1 1156-1448 bp 0.990476 91.2
petN-psbM_p2 ATCCGGAAAAAGTTGCACCT CGGCGGGTTGATTTGATAGA 45.0 50.0 58.0 58.1 1478-1629 bp 0.590476 74.1
petN-psbM_p3 ACTCGTAGTGTGGGGAAGAA TATTCAAGTCTTGACGGCGG 50.0 50.0 58.0 58.0 1281-1320 bp 0.285714 57.2
petN-psbM_p4 TCGTAGTGTGGGGAAGAAGT TATTCAAGTCTTGACGGCGG 50.0 50.0 58.0 58.0 1279-1318 bp 0.285714 57.2
petN-psbM_p5 ATCCGGAAAAAGTTGCACCT TATTCAAGTCTTGACGGCGG 45.0 50.0 58.0 58.0 1601-1629 bp 0.171429 49.1