Hotspot

atpH-atpI

LSC · 13283-14293 · 1011 bp

Back to Salvia

Pi 0.0315
MarkerSeek score 70.50
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1011
variable_sites
228
pi
0.0315
unique_haplotype_count
86
primer_available
yes
alignment_reliability
0.97
markerseek_score
70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AACAACTCACACACACTCCC TTCGGAACCCACAAACCATT 50.0 45.0 58.0 57.9 2156-2305 bp 0.971429 90.0
atpH-atpI_p2 AACAACTCACACACACTCCC GCAGATAGGGGGTTTCCAAG 50.0 55.0 58.0 57.9 2245-2394 bp 0.542857 72.9
atpH-atpI_p3 CACACACACTCCCTTTCCAA GCAGATAGGGGGTTTCCAAG 50.0 55.0 57.9 57.9 2238-2387 bp 0.542857 72.8
atpH-atpI_p4 AACAACTCACACACACTCCC TGCTTTGGCTTTACTCACGT 50.0 45.0 58.0 58.0 2003-2041 bp 0.333333 61.9
atpH-atpI_p5 CACACACACTCCCTTTCCAA TGCTTTGGCTTTACTCACGT 50.0 45.0 57.9 58.0 1996-2034 bp 0.333333 61.8