Hotspot

ycf1

SSC · 123715-129075 · 5361 bp

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Pi 0.0572

MarkerSeek score 74.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5361
variable_sites: 742
pi: 0.0572
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTTCCGGACTCAATTCGTG	AATCAACATTGAGGCCTGGG	50.0	50.0	57.9	58.1	672 bp	0.666667	65.5
ycf1_p2	CCCATAGTCTCACCCCATCT	AATCAACATTGAGGCCTGGG	55.0	50.0	57.9	58.1	561 bp	0.666667	65.2
ycf1_p3	TCGCAATTCTCTACGACGTC	AATCAACATTGAGGCCTGGG	50.0	50.0	57.8	58.1	931 bp	0.666667	64.8
ycf1_p4	CGCAATTCTCTACGACGTCT	AATCAACATTGAGGCCTGGG	50.0	50.0	57.8	58.1	930 bp	0.666667	64.8
ycf1_p5	ACCCATAGTCTCACCCCATC	AATCAACATTGAGGCCTGGG	55.0	50.0	58.2	58.1	562 bp	0.666667	64.6