Hotspot

ycf1-trnN-GUU

IRa · 129076-129590 · 515 bp

Back to Salsola

Pi 0.0493

MarkerSeek score 71.60

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 515
variable_sites: 62
pi: 0.0493
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	AACCTGAGTTCGGAGAAGGA	CGGGAGAATCAATGACTGCA	50.0	50.0	58.0	58.0	882-1002 bp	1.0	90.5
ycf1-trnN-GUU_p2	ACCTGAGTTCGGAGAAGGAA	CGGGAGAATCAATGACTGCA	50.0	50.0	58.0	58.0	881-1001 bp	1.0	90.5
ycf1-trnN-GUU_p3	CGGAGAAGGAAGAAATGCGA	CGGGAGAATCAATGACTGCA	50.0	50.0	58.0	58.0	929-992 bp	0.666667	70.6
ycf1-trnN-GUU_p4	TCGGAGAAGGAAGAAATGCG	CGGGAGAATCAATGACTGCA	50.0	50.0	58.0	58.0	930-993 bp	0.666667	70.6
ycf1-trnN-GUU_p5	CCGTAACGACCGAATTGACT	CGGGAGAATCAATGACTGCA	50.0	50.0	58.0	58.0	865-928 bp	0.666667	70.6