Hotspot

trnN-GUU-ycf1

IRb · 107359-107873 · 515 bp

Back to Salsola

Pi 0.0494

MarkerSeek score 71.80

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: IRb
length_bp: 515
variable_sites: 62
pi: 0.0494
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	CGGGAGAATCAATGACTGCA	AACCTGAGTTCGGAGAAGGA	50.0	50.0	58.0	58.0	882-1002 bp	1.0	90.5
trnN-GUU-ycf1_p2	CGGGAGAATCAATGACTGCA	ACCTGAGTTCGGAGAAGGAA	50.0	50.0	58.0	58.0	881-1001 bp	1.0	90.5
trnN-GUU-ycf1_p3	CGGGAGAATCAATGACTGCA	CGGAGAAGGAAGAAATGCGA	50.0	50.0	58.0	58.0	929-992 bp	0.666667	70.6
trnN-GUU-ycf1_p4	CGGGAGAATCAATGACTGCA	TCGGAGAAGGAAGAAATGCG	50.0	50.0	58.0	58.0	930-993 bp	0.666667	70.6
trnN-GUU-ycf1_p5	CGGGAGAATCAATGACTGCA	CCGTAACGACCGAATTGACT	50.0	50.0	58.0	58.0	865-928 bp	0.666667	70.6