Hotspot

ndhC-trnV-UAC

LSC · 50504-51253 · 750 bp

Back to Salsola

Pi 0.0365
MarkerSeek score 70.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
750
variable_sites
68
pi
0.0365
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 AGCGGCCATAAACTTGAGAG AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.0 57.8 1315-1339 bp 1.0 86.2
ndhC-trnV-UAC_p2 GGGTCCTTTGCTATTCGGAG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 897-921 bp 0.888889 83.7
ndhC-trnV-UAC_p3 AGCGGCCATAAACTTGAGAG GGAGCATAAACACAAGGGCT 50.0 50.0 58.0 58.2 1967-1985 bp 0.888889 80.2
ndhC-trnV-UAC_p4 TGGTTCAGGCATTTGCTCAT GGAGCATAAACACAAGGGCT 45.0 50.0 58.1 58.2 2154-2172 bp 0.888889 79.5
ndhC-trnV-UAC_p5 GGGTCCTTTGCTATTCGGAG GGAGCATAAACACAAGGGCT 55.0 50.0 58.0 58.2 1549-1566 bp 0.777778 76.1