Hotspot

rps4-trnT-UGU

LSC · 45835-46268 · 434 bp

Back to Salsola

Pi 0.0527

MarkerSeek score 78.90

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 434
variable_sites: 50
pi: 0.0527
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AGTCCAGGTAAAGCCCCTAG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.1	57.9	535-567 bp	1.0	89.2
rps4-trnT-UGU_p2	GTCCAGGTAAAGCCCCTAGA	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.1	57.9	534-566 bp	1.0	89.2
rps4-trnT-UGU_p3	TCCAGGTAAAGCCCCTAGAC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.1	57.9	533-565 bp	1.0	89.2
rps4-trnT-UGU_p4	AGTCCAGGTAAAGCCCCTAG	ATGGTCATCGGTTCGATTCC	55.0	50.0	58.1	57.8	503-535 bp	1.0	88.8
rps4-trnT-UGU_p5	TCCAGGTAAAGCCCCTAGAC	ATGGTCATCGGTTCGATTCC	55.0	50.0	58.1	57.8	501-533 bp	1.0	88.8