Hotspot

trnT-GGU-psbD

LSC · 31110-32409 · 1300 bp

Back to Salsola

Pi 0.0297
MarkerSeek score 71.70
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1300
variable_sites
102
pi
0.0297
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG CGTAACCAGTCATCCATAACA 55.0 42.9 58.1 55.4 1438-1483 bp 1.0 60.5
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA CGTAACCAGTCATCCATAACA 50.0 42.9 58.1 55.4 1441-1486 bp 1.0 60.4
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA CGTAACCAGTCATCCATAACA 50.0 42.9 58.3 55.4 1415-1460 bp 1.0 58.9
trnT-GGU-psbD_p4 CCGATAAGGGGCTTTTGAGG CGTAACCAGTCATCCATAACA 55.0 42.9 58.3 55.4 1393-1438 bp 1.0 58.7
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG CGTAACCAGTCATCCATAACATC 55.0 43.5 58.1 57.1 1438-1483 bp 1.0 57.9