Hotspot

trnK-UUU-rps16

LSC · 4199-4799 · 601 bp

Back to Salsola

Pi 0.0501

MarkerSeek score 74.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 601
variable_sites: 81
pi: 0.0501
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	TCTATCCCAATGAGCCGTCT	55.0	50.0	58.0	57.9	938-1066 bp	1.0	90.2
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	AGCCGTCTATCGAATCGTTG	55.0	50.0	58.0	57.9	926-1054 bp	1.0	90.1
trnK-UUU-rps16_p3	TCAGGATCAGTCGTGGTCTT	TCTATCCCAATGAGCCGTCT	50.0	50.0	58.1	57.9	934-1062 bp	1.0	89.8
trnK-UUU-rps16_p4	CCTTTCAGGATCAGTCGTGG	TTGCGGCCATTTTTGAAAGG	55.0	45.0	58.0	58.1	1407-1529 bp	1.0	89.5
trnK-UUU-rps16_p5	CCTTTCAGGATCAGTCGTGG	TCCGTTGTTCGCATGATTCT	55.0	45.0	58.0	57.9	1462-1476 bp	0.666667	66.1