Hotspot

accD

Genome · 77049-80477 · 3429 bp

Back to Salmonopuntia

Pi 0.00786

MarkerSeek score 50.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: Genome
length_bp: 3429
variable_sites: 26
pi: 0.00786
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TTCTGGATATTCAGCACGCC	CTGCGGACTCTTCTTCCTTG	50.0	55.0	58.0	58.3	311 bp	1.0	78.7
accD_p2	TTCTGGATATTCAGCACGCC	TCTGCGGACTCTTCTTCCTT	50.0	50.0	58.0	58.4	312 bp	1.0	78.0
accD_p3	TTCTGGATATTCAGCACGCC	TTCTGCGGACTCTTCTTCCT	50.0	50.0	58.0	58.4	313 bp	1.0	78.0
accD_p4	CACGCCGAATGGATGGTATT	CTGCGGACTCTTCTTCCTTG	50.0	55.0	58.4	58.3	297 bp	1.0	75.8
accD_p5	TTCTGGATATTCAGCACGCC	ATTCTGCGGACTCTTCTTCC	50.0	50.0	58.0	57.3	314 bp	1.0	75.4