Hotspot

trnN-GUU-trnR-ACG

Genome · 138535-139156 · 622 bp

Back to Salmonopuntia

Pi 0
MarkerSeek score 62.50
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
Genome
length_bp
622
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 CCGTTGTTCCTCAGTAGCTC CAGAGGATTAGAGCACGTGG 55.0 55.0 58.0 57.8 739-763 bp 1.0 80.8
trnN-GUU-trnR-ACG_p2 GATCGAGTCTCCCGTTGTTC CAGAGGATTAGAGCACGTGG 55.0 55.0 58.1 57.8 750-774 bp 1.0 80.1
trnN-GUU-trnR-ACG_p3 TGGGTTGAGAACGGGAATTG CAGAGGATTAGAGCACGTGG 50.0 55.0 58.1 57.8 780-804 bp 1.0 79.9
trnN-GUU-trnR-ACG_p4 TGGTCGTAGGTTCGAATCCT CAGAGGATTAGAGCACGTGG 50.0 55.0 58.2 57.8 690-714 bp 1.0 79.8
trnN-GUU-trnR-ACG_p5 GGGTTGAGAACGGGAATTGA CAGAGGATTAGAGCACGTGG 50.0 55.0 57.8 57.8 779-803 bp 1.0 79.1