Hotspot

trnL-UAG-ccsA

Genome · 121067-121180 · 114 bp

Back to Salmonopuntia

Pi 0.0263

MarkerSeek score 65.20

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: Genome
length_bp: 114
variable_sites: 3
pi: 0.0263
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	CTCTTAGGAAGCAGTGCGAG	TGGAAACCATTGAAAAGGAA	55.0	35.0	58.1	53.1	222-228 bp	1.0	41.5
trnL-UAG-ccsA_p2	CTTAGGAAGCAGTGCGAGAG	TGGAAACCATTGAAAAGGAA	55.0	35.0	58.1	53.1	220-226 bp	1.0	41.5
trnL-UAG-ccsA_p3	TAGACACGCTGCTCTTAGGA	TGGAAACCATTGAAAAGGAA	50.0	35.0	57.9	53.1	233-239 bp	1.0	41.1
trnL-UAG-ccsA_p4	CTCTTAGGAAGCAGTGCGAG	TGGAAACCATTGAAAAGGAAA	55.0	33.3	58.1	54.1	222-228 bp	1.0	41.1
trnL-UAG-ccsA_p5	CTTAGGAAGCAGTGCGAGAG	TGGAAACCATTGAAAAGGAAA	55.0	33.3	58.1	54.1	220-226 bp	1.0	41.1