Hotspot

ndhA-trnL-UAG

Genome · 120168-120986 · 819 bp

Back to Salmonopuntia

Pi 0.00122

MarkerSeek score 54.70

Primer available yes

Label ndhA-trnL-UAG

Feature Metadata

label_name: ndhA-trnL-UAG
feature_type: igs
region: Genome
length_bp: 819
variable_sites: 1
pi: 0.00122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA-trnL-UAG_p1	TTTTAGGTGGTCTACGGGCT	CTCGCACTGCTTCCTAAGAG	50.0	55.0	58.4	58.1	964-992 bp	1.0	76.7
ndhA-trnL-UAG_p2	TTTTAGGTGGTCTACGGGCT	CTCTCGCACTGCTTCCTAAG	50.0	55.0	58.4	58.1	966-994 bp	1.0	76.7
ndhA-trnL-UAG_p3	TTTTAGGTGGTCTACGGGCT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.4	57.9	953-981 bp	1.0	76.4
ndhA-trnL-UAG_p4	TTTTAGGTGGTCTACGGGCT	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.4	57.8	946-974 bp	1.0	75.9
ndhA-trnL-UAG_p5	TTTTAGGTGGTCTACGGGCT	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.4	57.8	951-979 bp	1.0	75.8