Hotspot

trnR-ACG-trnN-GUU

Genome · 109230-109851 · 622 bp

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Pi 0
MarkerSeek score 62.50
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
Genome
length_bp
622
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 CAGAGGATTAGAGCACGTGG CCGTTGTTCCTCAGTAGCTC 55.0 55.0 57.8 58.0 739-763 bp 1.0 80.8
trnR-ACG-trnN-GUU_p2 CAGAGGATTAGAGCACGTGG GATCGAGTCTCCCGTTGTTC 55.0 55.0 57.8 58.1 750-774 bp 1.0 80.1
trnR-ACG-trnN-GUU_p3 CAGAGGATTAGAGCACGTGG TGGGTTGAGAACGGGAATTG 55.0 50.0 57.8 58.1 780-804 bp 1.0 79.9
trnR-ACG-trnN-GUU_p4 CAGAGGATTAGAGCACGTGG TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.8 58.2 690-714 bp 1.0 79.8
trnR-ACG-trnN-GUU_p5 CAGAGGATTAGAGCACGTGG GGGTTGAGAACGGGAATTGA 55.0 50.0 57.8 57.8 779-803 bp 1.0 79.1