Hotspot

accD-psaI

Genome · 80478-81098 · 621 bp

Back to Salmonopuntia

Pi 0.00484

MarkerSeek score 61.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: Genome
length_bp: 621
variable_sites: 3
pi: 0.00484
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TACTTGAGCTGCACGGTTTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	744 bp	1.0	79.9
accD-psaI_p2	TTACTTGAGCTGCACGGTTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	745 bp	1.0	79.9
accD-psaI_p3	TACTTGAGCTGCACGGTTTT	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.0	57.9	750 bp	1.0	79.3
accD-psaI_p4	TTACTTGAGCTGCACGGTTT	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.0	57.9	751 bp	1.0	79.3
accD-psaI_p5	TCGATCCAGTCTTACCACGT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	792 bp	1.0	78.8