Hotspot

trnR-UCU-atpA

Genome · 28319-28419 · 101 bp

Back to Salmonopuntia

Pi 0.0495

MarkerSeek score 81.00

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: Genome
length_bp: 101
variable_sites: 5
pi: 0.0495
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ATCGGAAAAGCGTCCATTGT	GAGGAAGCAGAAGCCCTTTT	45.0	50.0	58.2	58.1	257 bp	1.0	79.5
trnR-UCU-atpA_p2	CGGAAAAGCGTCCATTGTCT	GAGGAAGCAGAAGCCCTTTT	50.0	50.0	58.8	58.1	255 bp	1.0	74.2
trnR-UCU-atpA_p3	TCGGAAAAGCGTCCATTGTC	GAGGAAGCAGAAGCCCTTTT	50.0	50.0	58.8	58.1	256 bp	1.0	74.1
trnR-UCU-atpA_p4	ATCGGAAAAGCGTCCATTGT	TTACTGAGGAAGCAGAAGCC	45.0	50.0	58.2	57.2	262 bp	1.0	73.9
trnR-UCU-atpA_p5	AATCGGAAAAGCGTCCATTG	GAGGAAGCAGAAGCCCTTTT	45.0	50.0	57.1	58.1	258 bp	1.0	73.5