Hotspot

rpl23-rpl2

Genome · 12956-13021 · 66 bp

Back to Salmonopuntia

Pi 0.0152

MarkerSeek score 61.20

Primer available yes

Label rpl23-rpl2

Feature Metadata

label_name: rpl23-rpl2
feature_type: igs
region: Genome
length_bp: 66
variable_sites: 1
pi: 0.0152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-rpl2_p1	TCATTACGCTTCAATCGGGT	TGCTCGGGGTAGAAGTTTTG	45.0	50.0	57.3	57.8	144 bp	1.0	73.7
rpl23-rpl2_p2	TCATTACGCTTCAATCGGGT	GCTCGGGGTAGAAGTTTTGT	45.0	50.0	57.3	57.8	143 bp	1.0	73.6
rpl23-rpl2_p3	TCATTACGCTTCAATCGGGT	GTGCTCGGGGTAGAAGTTTT	45.0	50.0	57.3	57.8	145 bp	1.0	73.6
rpl23-rpl2_p4	TCATTACGCTTCAATCGGGT	CAACGACGCTGTCCATAGAT	45.0	50.0	57.3	57.8	217 bp	1.0	73.2
rpl23-rpl2_p5	TCATTACGCTTCAATCGGGT	ATTGTCTACGGCTCCATTGC	45.0	50.0	57.3	58.3	167 bp	1.0	72.4