Hotspot

ndhA

SSC · 124224-126426 · 2203 bp

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Pi 0.0072
MarkerSeek score 47.50
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2203
variable_sites
73
pi
0.0072
unique_haplotype_count
39
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 TGTTTCTTTATCTTGTTTGAGACA ATGGGGGAAGTTGATCGTTG 29.2 50.0 54.9 57.9 2256-2288 bp 1.0 44.9
ndhA_p2 TGTTTCTTTATCTTGTTTGAGACA GGGGGAAGTTGATCGTTGAA 29.2 50.0 54.9 57.8 2254-2286 bp 1.0 44.9
ndhA_p3 TCTTTATCTTGTTTGAGACAAACT ATGGGGGAAGTTGATCGTTG 29.2 50.0 54.7 57.9 2252-2284 bp 1.0 44.9
ndhA_p4 TGTTTCTTTATCTTGTTTGAGACAA ATGGGGGAAGTTGATCGTTG 28.0 50.0 55.6 57.9 2256-2288 bp 1.0 44.9
ndhA_p5 TTGTTTCTTTATCTTGTTTGAGACA ATGGGGGAAGTTGATCGTTG 28.0 50.0 55.6 57.9 2257-2289 bp 1.0 44.9