Hotspot

ccsA

SSC · 118843-119805 · 963 bp

Pi 0.00628

MarkerSeek score 35.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 963
variable_sites: 28
pi: 0.00628
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	TCAACGGGGCCATTTTACAA	50.0	45.0	58.0	58.0	1855-1879 bp	1.0	83.0
ccsA_p2	TGAAATCGGTAGACACGCTG	TCAACGGGGCCATTTTACAA	50.0	45.0	58.0	58.0	1854-1878 bp	1.0	83.0
ccsA_p3	GTGAAATCGGTAGACACGCT	TTACTTTTGGAGCGGCAGTT	50.0	45.0	58.0	58.0	1585-1609 bp	1.0	83.0
ccsA_p4	TGAAATCGGTAGACACGCTG	TTACTTTTGGAGCGGCAGTT	50.0	45.0	58.0	58.0	1584-1608 bp	1.0	82.9
ccsA_p5	GTGAAATCGGTAGACACGCT	AGCTTCTCTTGCATTACCGG	50.0	50.0	58.0	58.0	1689-1712 bp	0.932203	79.7