Hotspot

trnN-GUU-trnR-ACG

IRa · 134250-134975 · 726 bp

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Pi 0.00442
MarkerSeek score 56.40
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
726
variable_sites
23
pi
0.00442
unique_haplotype_count
20
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 CCGTTGTTCCTCAGTAGCTC AACAGATCCAGTGGAGACGA 55.0 50.0 58.0 58.1 752-1017 bp 1.0 85.4
trnN-GUU-trnR-ACG_p2 CCGTTGTTCCTCAGTAGCTC TAGGGTATTCCGGGGAACAG 55.0 55.0 58.0 58.2 767-1032 bp 1.0 84.3
trnN-GUU-trnR-ACG_p3 CCGTTGTTCCTCAGTAGCTC CAGAGGATTAGAGCACGTGG 55.0 55.0 58.0 57.8 716-981 bp 1.0 84.3
trnN-GUU-trnR-ACG_p4 GCTGTTAACTGACTGGTCGT AACAGATCCAGTGGAGACGA 50.0 50.0 57.8 58.1 716-981 bp 1.0 84.3
trnN-GUU-trnR-ACG_p5 TGGTCGTAGGTTCGAATCCT AACAGATCCAGTGGAGACGA 50.0 50.0 58.2 58.1 703-968 bp 1.0 84.3