Hotspot

rps15-ycf1

IRa · 127998-128411 · 414 bp

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Pi 0.0257
MarkerSeek score 57.90
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
IRa
length_bp
414
variable_sites
45
pi
0.0257
unique_haplotype_count
20
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TGAAATGAATGCACTTTTTACCA AGTCGGTTTAGTATGGTAAGGA 30.4 40.9 55.6 56.0 167-501 bp 1.0 51.6
rps15-ycf1_p2 TGAAATGAATGCACTTTTTACCA TGTCCACGATTAAAAATTCGGT 30.4 36.4 55.6 56.6 137-471 bp 0.983051 51.0
rps15-ycf1_p3 TTGAAATGAATGCACTTTTTACCA TGTCCACGATTAAAAATTCGGT 29.2 36.4 56.3 56.6 138-472 bp 0.983051 51.0
rps15-ycf1_p4 TGAAATGAATGCACTTTTTACCAT TGTCCACGATTAAAAATTCGGT 29.2 36.4 56.1 56.6 137-471 bp 0.983051 51.0
rps15-ycf1_p5 TGAAATGAATGCACTTTTTACCA ATGTCCACGATTAAAAATTCGGT 30.4 34.8 55.6 57.1 138-472 bp 0.983051 51.0