Hotspot

petA-psbJ

LSC · 62752-63613 · 862 bp

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Pi 0.00886
MarkerSeek score 63.30
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
862
variable_sites
45
pi
0.00886
unique_haplotype_count
28
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCCTTTTGTTCTTCTTGGC GCTGGTATTCCTGTGATCGG 50.0 55.0 57.8 58.1 1031-1062 bp 0.983051 84.7
petA-psbJ_p2 CATTACGTGTCCAAGGCCTT GCTGGTATTCCTGTGATCGG 50.0 55.0 58.2 58.1 1045-1076 bp 0.983051 84.4
petA-psbJ_p3 GGCCTTTTGTTCTTCTTGGC TATGGCTCCTATTCCGGCTT 50.0 50.0 57.8 58.3 992-1023 bp 1.0 84.3
petA-psbJ_p4 GGCCTTTTGTTCTTCTTGGC TTATGGCTCCTATTCCGGCT 50.0 50.0 57.8 58.3 993-1024 bp 1.0 84.3
petA-psbJ_p5 GACCCATTACGTGTCCAAGG GCTGGTATTCCTGTGATCGG 55.0 55.0 58.3 58.1 1049-1080 bp 0.983051 83.7