Hotspot

rps4-trnT-UGU

LSC · 45714-45943 · 230 bp

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Pi 0.0137
MarkerSeek score 57.80
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
230
variable_sites
18
pi
0.0137
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 GCGCGCGATTGATTTCTAAG CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.3 57.9 377-408 bp 1.0 83.7
rps4-trnT-UGU_p2 GCGCGCGATTGATTTCTAAG GGTTCGATTCCGATAGCTGG 50.0 55.0 58.3 58.2 336-367 bp 1.0 83.5
rps4-trnT-UGU_p3 GCGCGCGATTGATTTCTAAG ATGGTCATCGGTTCGATTCC 50.0 50.0 58.3 57.8 345-376 bp 1.0 83.2
rps4-trnT-UGU_p4 GCGCGCGATTGATTTCTAAG TGTTATGTGAGCCCGCTTAG 50.0 50.0 58.3 57.7 397-428 bp 1.0 82.2
rps4-trnT-UGU_p5 GCGCGCGATTGATTTCTAAG CTGTTATGTGAGCCCGCTTA 50.0 50.0 58.3 57.7 398-429 bp 1.0 82.1