Hotspot

petN-psbM

LSC · 27205-28517 · 1313 bp

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Pi 0.00447
MarkerSeek score 53.20
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1313
variable_sites
42
pi
0.00447
unique_haplotype_count
27
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 1228-1496 bp 1.0 73.8
petN-psbM_p2 CTTGGGCTGCTTTGATGGTA TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 1216-1484 bp 1.0 73.8
petN-psbM_p3 TTGGGCTGCTTTGATGGTAG TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 1215-1483 bp 1.0 73.8
petN-psbM_p4 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.6 57.6 1167-1435 bp 1.0 72.3
petN-psbM_p5 GGCTGCTTTGATGGTAGTCT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.6 57.6 1212-1480 bp 1.0 71.8