Hotspot

psbE-petL

LSC · 64239-65579 · 1341 bp

Back to Salicornia

Pi 0.00962

MarkerSeek score 61.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1341
variable_sites: 28
pi: 0.00962
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AGCGGAGGTAATAGTTAAAGCC	45.0	45.5	58.0	57.9	1471-1516 bp	1.0	68.8
psbE-petL_p2	TGACAAATAACCAACCCGCA	GGAGGTAATAGTTAAAGCCGCT	45.0	45.5	58.0	57.9	1468-1513 bp	1.0	68.8
psbE-petL_p3	CAACCCGCAATGAATAGGGA	AGCGGAGGTAATAGTTAAAGCC	50.0	45.5	57.9	57.9	1460-1505 bp	1.0	68.5
psbE-petL_p4	CAACCCGCAATGAATAGGGA	GGAGGTAATAGTTAAAGCCGCT	50.0	45.5	57.9	57.9	1457-1502 bp	1.0	68.5
psbE-petL_p5	AACCCGCAATGAATAGGGAC	GGAGGTAATAGTTAAAGCCGCT	50.0	45.5	57.9	57.9	1456-1501 bp	1.0	68.5