Hotspot

ycf4-cemA

LSC · 59763-60497 · 735 bp

Back to Salicornia

Pi 0.0142

MarkerSeek score 59.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 25
pi: 0.0142
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CCTTGACTCGTACGGATGAG	AGAGAGATCCACCAGGGTAA	55.0	50.0	57.5	56.7	897-916 bp	1.0	70.7
ycf4-cemA_p2	CCTTGACTCGTACGGATGAG	TGATAGAGAGATCCACCAGGG	55.0	52.4	57.5	57.7	901-920 bp	1.0	70.7
ycf4-cemA_p3	CCTTGACTCGTACGGATGAG	GATAGAGAGATCCACCAGGGT	55.0	52.4	57.5	57.7	900-919 bp	1.0	70.7
ycf4-cemA_p4	CCTTGACTCGTACGGATGAG	AGTAACCCAAGACTCCAGACT	55.0	47.6	57.5	57.7	931-950 bp	1.0	70.4
ycf4-cemA_p5	CCTTGACTCGTACGGATGAG	ATAGAGAGATCCACCAGGGT	55.0	50.0	57.5	56.5	899-918 bp	1.0	68.8