Hotspot

accD-psaI

LSC · 58080-58763 · 684 bp

Back to Salicornia

Pi 0.014

MarkerSeek score 60.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 684
variable_sites: 22
pi: 0.014
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AACGCCTACATTGCATTTGC	ACGTTCTGATCGCCAATTCA	45.0	45.0	58.0	57.9	1341-1361 bp	1.0	84.4
accD-psaI_p2	ACCGCCAGTTTTGGTATGTT	ACGTTCTGATCGCCAATTCA	45.0	45.0	58.0	57.9	1386-1406 bp	1.0	84.2
accD-psaI_p3	GCATGCAAGAAGGAAGCTTG	ACGTTCTGATCGCCAATTCA	50.0	45.0	58.0	57.9	1511-1531 bp	1.0	83.9
accD-psaI_p4	AACGCCTACATTGCATTTGC	GAAAGACATCACGAGCCCTT	45.0	50.0	58.0	57.9	1524-1544 bp	1.0	83.8
accD-psaI_p5	GCATGCAAGAAGGAAGCTTG	GAAAGACATCACGAGCCCTT	50.0	50.0	58.0	57.9	1694-1714 bp	1.0	83.4