Hotspot

ndhC-ACI62_gt018

LSC · 49959-50636 · 678 bp

Back to Salicornia

Pi 0.0121
MarkerSeek score 63.50
Primer available yes
Label ndhC-ACI62_gt018

Feature Metadata

label_name
ndhC-ACI62_gt018
feature_type
igs
region
LSC
length_bp
678
variable_sites
20
pi
0.0121
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-ACI62_gt018_p1 CGGGTCCTTTGTTATTCGGA TGCCTGTTGGGTCTTTGAAA 50.0 45.0 57.6 57.8 891-902 bp 1.0 78.1
ndhC-ACI62_gt018_p2 TCGGGTCCTTTGTTATTCGG TGCCTGTTGGGTCTTTGAAA 50.0 45.0 57.6 57.8 892-903 bp 1.0 78.1
ndhC-ACI62_gt018_p3 CGGGTCCTTTGTTATTCGGA AAAAAGTGCCTGTTGGGTCT 50.0 45.0 57.6 57.8 897-908 bp 1.0 78.0
ndhC-ACI62_gt018_p4 CGGGTCCTTTGTTATTCGGA AAAAGTGCCTGTTGGGTCTT 50.0 45.0 57.6 57.8 896-907 bp 1.0 78.0
ndhC-ACI62_gt018_p5 CGGGTCCTTTGTTATTCGGA AAAGTGCCTGTTGGGTCTTT 50.0 45.0 57.6 57.8 895-906 bp 1.0 78.0