Hotspot

ACI62_gt017-ndhJ

LSC · 47544-48247 · 704 bp

Back to Salicornia

Pi 0.0118
MarkerSeek score 65.80
Primer available yes
Label ACI62_gt017-ndhJ

Feature Metadata

label_name
ACI62_gt017-ndhJ
feature_type
igs
region
LSC
length_bp
704
variable_sites
20
pi
0.0118
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ACI62_gt017-ndhJ_p1 CCTCGTGTCACCAGTTCAAA TAATCATCCACGCCTGAAGC 50.0 50.0 58.1 58.0 843-848 bp 1.0 82.5
ACI62_gt017-ndhJ_p2 CCTCGTGTCACCAGTTCAAA AGTTGGATAGGATGGCCCTT 50.0 50.0 58.1 58.1 806-811 bp 1.0 82.3
ACI62_gt017-ndhJ_p3 AATGACTGGTCGGGATAGCT TAATCATCCACGCCTGAAGC 50.0 50.0 58.2 58.0 890-895 bp 1.0 81.1
ACI62_gt017-ndhJ_p4 AATGACTGGTCGGGATAGCT AGTTGGATAGGATGGCCCTT 50.0 50.0 58.2 58.1 853-858 bp 1.0 80.9
ACI62_gt017-ndhJ_p5 CCTCGTGTCACCAGTTCAAA ATGCCCGAAAGTTGGATAGG 50.0 50.0 58.1 57.7 815-820 bp 1.0 80.2