Hotspot

ACI62_gt015-ACI62_gt016

LSC · 45906-46483 · 578 bp

Back to Salicornia

Pi 0.0197

MarkerSeek score 56.10

Primer available yes

Label ACI62_gt015-ACI62_gt016

Feature Metadata

label_name: ACI62_gt015-ACI62_gt016
feature_type: igs
region: LSC
length_bp: 578
variable_sites: 21
pi: 0.0197
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ACI62_gt015-ACI62_gt016_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	570-639 bp	1.0	83.8
ACI62_gt015-ACI62_gt016_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	602-671 bp	1.0	82.8
ACI62_gt015-ACI62_gt016_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	564-633 bp	1.0	82.5
ACI62_gt015-ACI62_gt016_p4	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	50.0	50.0	57.8	57.6	596-665 bp	1.0	81.5
ACI62_gt015-ACI62_gt016_p5	CTAACCTCTGAGCTAAGCGG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.5	58.2	562-631 bp	1.0	80.9