Hotspot

psbM-ACI62_gt007

LSC · 28703-29523 · 821 bp

Back to Salicornia

Pi 0.0127
MarkerSeek score 64.20
Primer available yes
Label psbM-ACI62_gt007

Feature Metadata

label_name
psbM-ACI62_gt007
feature_type
igs
region
LSC
length_bp
821
variable_sites
25
pi
0.0127
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-ACI62_gt007_p1 AGAATGAATAGCGCAGTAGCA TCAATTGGTTAGAGCACCGC 42.9 50.0 57.5 58.6 930-935 bp 1.0 69.7
psbM-ACI62_gt007_p2 GAATGAATAGCGCAGTAGCA TCAATTGGTTAGAGCACCGC 45.0 50.0 56.0 58.6 929-934 bp 1.0 65.6
psbM-ACI62_gt007_p3 AGAATGAATAGCGCAGTAGCA AATTGGTTAGAGCACCGCC 42.9 52.6 57.5 58.1 928-933 bp 1.0 65.0
psbM-ACI62_gt007_p4 AGAATGAATAGCGCAGTAGCAA TCAATTGGTTAGAGCACCGC 40.9 50.0 58.2 58.6 930-935 bp 1.0 63.7
psbM-ACI62_gt007_p5 GAATGAATAGCGCAGTAGCAA TCAATTGGTTAGAGCACCGC 42.9 50.0 56.8 58.6 929-934 bp 1.0 63.6