Hotspot

rpoB-ACI62_gt006

LSC · 26424-27605 · 1182 bp

Back to Salicornia

Pi 0.0235

MarkerSeek score 71.90

Primer available yes

Label rpoB-ACI62_gt006

Feature Metadata

label_name: rpoB-ACI62_gt006
feature_type: igs
region: LSC
length_bp: 1182
variable_sites: 59
pi: 0.0235
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-ACI62_gt006_p1	ATTCCCTCATTTCCATCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	1245-1282 bp	1.0	81.8
rpoB-ACI62_gt006_p2	TTCCCTCATTTCCATCCCGT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.7	58.1	1244-1281 bp	1.0	81.5
rpoB-ACI62_gt006_p3	ATTCCCTCATTTCCATCCCG	AAAGGATTTGCAGTCCTCCG	50.0	50.0	57.3	58.2	1226-1263 bp	1.0	81.1
rpoB-ACI62_gt006_p4	TTCCCTCATTTCCATCCCGT	AAAGGATTTGCAGTCCTCCG	50.0	50.0	58.7	58.2	1225-1262 bp	1.0	80.8
rpoB-ACI62_gt006_p5	CCTCATTTCCATCCCGTCG	CCGGATTTGAACTGGGGAAA	57.9	50.0	58.0	58.1	1241-1278 bp	1.0	79.0