Hotspot

ycf1

SSC · 129568-135270 · 5703 bp

Back to Salacia

Pi 0.00701

MarkerSeek score 44.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5703
variable_sites: 60
pi: 0.00701
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATGAGTCCTTCGGATCTTCA	AGTACCCCAAAAATGGCGAA	45.0	45.0	55.7	57.7	200-224 bp	1.0	61.8
ycf1_p2	TGAGTCCTTCGGATCTTCAT	AGTACCCCAAAAATGGCGAA	45.0	45.0	55.7	57.7	199-223 bp	1.0	61.8
ycf1_p3	ATGAGTCCTTCGGATCTTCA	CCCCAAAAATGGCGAAATGT	45.0	45.0	55.7	57.5	196-220 bp	1.0	60.5
ycf1_p4	TGAGTCCTTCGGATCTTCAT	ACCCCAAAAATGGCGAAATG	45.0	45.0	55.7	57.5	196-220 bp	1.0	60.5
ycf1_p5	TGAGTCCTTCGGATCTTCAT	CCCCAAAAATGGCGAAATGT	45.0	45.0	55.7	57.5	195-219 bp	1.0	60.5