Hotspot

ndhJ-ndhK

LSC · 52242-52375 · 134 bp

Back to Salacia

Pi 0.0274

MarkerSeek score 73.30

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 134
variable_sites: 4
pi: 0.0274
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	GAGCAGACAAACAACCTTGC	ACACAGCAGGGTCAGTATCT	50.0	50.0	57.9	58.1	170-203 bp	1.0	84.3
ndhJ-ndhK_p2	TGAACTAGCCCGTGTTTGAC	ACACAGCAGGGTCAGTATCT	50.0	50.0	58.1	58.1	195-228 bp	1.0	84.1
ndhJ-ndhK_p3	TCCGCGGGCTTTATTTGTAA	ACACAGCAGGGTCAGTATCT	45.0	50.0	57.9	58.1	252-285 bp	1.0	83.9
ndhJ-ndhK_p4	CCTTGGTAATCGAAGCCCAA	ACACAGCAGGGTCAGTATCT	50.0	50.0	57.9	58.1	222-255 bp	1.0	83.9
ndhJ-ndhK_p5	CAATCCGCGGGCTTTATTTG	ACACAGCAGGGTCAGTATCT	50.0	50.0	58.2	58.1	255-288 bp	1.0	83.4