Hotspot

trnF-GAA-ndhJ

LSC · 50881-51764 · 884 bp

Back to Salacia

Pi 0.00858

MarkerSeek score 65.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 884
variable_sites: 10
pi: 0.00858
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	CCCGAAAGTTGGATAGGGTG	55.0	55.0	58.0	58.0	997-1032 bp	1.0	83.7
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	CCGAAAGTTGGATAGGGTGG	55.0	55.0	58.0	58.0	996-1031 bp	1.0	83.7
trnF-GAA-ndhJ_p3	GCATTGAGAATGGTCGGGAT	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.0	58.0	1012-1047 bp	1.0	83.5
trnF-GAA-ndhJ_p4	GCATTGAGAATGGTCGGGAT	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.0	58.0	1011-1046 bp	1.0	83.5
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	960-995 bp	1.0	83.5