Hotspot

psaA-ycf3

LSC · 43619-44313 · 695 bp

Back to Salacia

Pi 0.00576

MarkerSeek score 62.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 695
variable_sites: 6
pi: 0.00576
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	AAGCCGCCGAATATTGGAAA	50.0	45.0	57.9	58.2	862-870 bp	1.0	78.5
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCTGG	50.0	55.0	57.9	57.6	839-847 bp	1.0	77.7
psaA-ycf3_p3	TGTTCTTGAGAAATGGCCGG	AAGCCGCCGAATATTGGAAA	50.0	45.0	58.5	58.2	887-895 bp	1.0	75.7
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	GATCAAGCCGCCGAATATTG	50.0	50.0	57.9	57.4	866-874 bp	1.0	75.6
psaA-ycf3_p5	TTTTACTTCTGGTTCCGGCG	AAGCCGCCGAATATTGGAAA	50.0	45.0	58.5	58.2	815-823 bp	1.0	75.6