Hotspot

psbZ-trnG-GCC

LSC · 37510-38246 · 737 bp

Back to Salacia

Pi 0.0159
MarkerSeek score 67.90
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
737
variable_sites
14
pi
0.0159
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTTGCTTCTCCTGAGGGTTG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.7 57.8 796-1019 bp 1.0 81.7
psbZ-trnG-GCC_p2 TGAGGGTTGGTCGAGTAACA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.3 57.8 785-1008 bp 1.0 81.6
psbZ-trnG-GCC_p3 CTGAGGGTTGGTCGAGTAAC CGTCTTCTCCTTGGCAAAGA 55.0 50.0 57.6 57.8 786-1009 bp 1.0 81.0
psbZ-trnG-GCC_p4 CCTGAGGGTTGGTCGAGTAA CGTCTTCTCCTTGGCAAAGA 55.0 50.0 58.7 57.8 787-1010 bp 1.0 78.0
psbZ-trnG-GCC_p5 GAGGGTTGGTCGAGTAACAA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.2 57.8 784-1007 bp 1.0 77.3