Hotspot

psbI-trnS-GGU

LSC · 7574-7812 · 239 bp

Back to Salacia

Pi 0.0292

MarkerSeek score 71.10

Primer available yes

Label psbI-trnS-GGU

Feature Metadata

label_name: psbI-trnS-GGU
feature_type: igs
region: LSC
length_bp: 239
variable_sites: 9
pi: 0.0292
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GGU_p1	AATCCCGGACGCGAAGAATA	GTTCTTCGTACCGAGGGTTC	50.0	55.0	59.3	58.0	282-296 bp	1.0	76.4
psbI-trnS-GGU_p2	ATCCCGGACGCGAAGAATAA	GTTCTTCGTACCGAGGGTTC	50.0	55.0	59.3	58.0	281-295 bp	1.0	76.4
psbI-trnS-GGU_p3	ATCCCGGACGCGAAGAATAA	CCTAAAGCGTCGGATTGCTA	50.0	50.0	59.3	57.8	314-328 bp	1.0	74.5
psbI-trnS-GGU_p4	AATCCCGGACGCGAAGAATA	ATTCGGAGAGATGGCTGAGT	50.0	50.0	59.3	58.2	337-351 bp	1.0	74.4
psbI-trnS-GGU_p5	ATCCCGGACGCGAAGAATAA	ATTCGGAGAGATGGCTGAGT	50.0	50.0	59.3	58.2	336-350 bp	1.0	74.4